题 目：Tailored Biology: Examination of Basal and Lipopolysaccharide-Induced Cellular Stress Response to Chemical and Topographical Stimuli from Biotic-Abiotic Functionalized Materials
报告人：Dean Ho Ph. D.
Assistant Professor， Departments of Biomedical and Mechanical Engineering， Northwestern University， Evanston, IL USA
联系人：澳门在线威尼斯官方生物医学工程系 马宏伟 (Tel. 6276-7142)
Tailored Biology: Examination of Basal and Lipopolysaccharide-Induced Cellular Stress Response to Chemical and Topographical Stimuli from Biotic-Abiotic Functionalized Materials
Serving as platforms for both cellular interrogation as well as biomembrane mimicry, biotic-abiotic functionalized materials such as copolymeric membranes offer the opportunity for
tailored biology, where specific embedded functionalities can be rapidly engineered, on demand, without the need for genetic processing. Reconstituting pore, or channel proteins, for example, into these copolymers transforms an otherwise non-biological component into a foundational matrix for the mimicry of active biological membranes. Envisioned
in vivo applications of this technology would include the utilization of ion-conduction proteins to mimic neural and cardiomyocyte membranes for regenerative medicine, as well as copolymer functionalization with cyto-effector molecules such as suppressors of cellular inflammation, as immuno-regulatory coatings for implants, etc. As such, these copolymeric membrane materials inspired by nature possess the potential to impact multiple medically relevant fields. An important element empowering this transition is the in-depth examination of the biocompatibility as well as bio-inert properties of these materials. This includes the interrogation of basal stress response of macrophages, or immune cells, to the chemical as well as topographical stimuli presented by these materials. We have developed copolymers functionalized with Dexamethasone (Dex), a glucocorticoid anti-inflammatory molecule, as well as the OmpF/BR proteins as representative membrane-based biomolecules, using the Langmuir-Blodgett deposition modality. Using surface pressure isotherms as well as fluorescence microscopy of Dex-FITC/copolymer complexes, we have confirmed the successful interface of the abiotic copolymer with the biotic protein and drug components. This technology successfully suppressed, to a significant degree, RAW264.7 macrophage transcriptional machinery of several cytokines including inducible nitric oxide synthase (iNOS), interleukin-6, and interleukin-12. These findings offer insight into both bio-abio material fabrication, as well as analysis of stress specific gene program induction by material functionality (e.g. Dex) and inherent properties (e.g. topography) which may be used to generate principles of biotic-abiotic interfacing for optimized biocompatibility and medical applications.
Dr. Dean Ho received his Ph.D. in 2005 from the UCLA Bioengineering Department. He was a Research Associate in the Departments of Electrical Engineering and Bioengineering at the California Institute of Technology as well as in the UCLA Mechanical and Aerospace Engineering Department from 2005-2006. He is currently an Assistant Professor in the Departments of Biomedical Engineering and Mechanical Engineering at Northwestern University where he directs the Laboratory for Nanoscale Biotic-Abiotic Systems Engineering (N-BASE).
His research has covered emerging areas of bio-nano-technology and protein-functionalized materials. Dr. Ho has been among the first to demonstrate the coupling of protein function with a polymer film to fabricate nano energy conversion systems. This work is the subject of his recently published article in
Nanotechnology (Cover Article) as well as a featured article in
Nanotechnology that was downloaded over 1000 times which was among the top 10% of all downloaded Institute of Physics (IOP) publications. He has published over 35 peer-reviewed journal and conference papers in the areas of bio/chemical energetics, biotic-abiotic interfacing, as well as developing novel solar power sources. In addition, Dr. Ho was an invited author of the highly visible publication,
Nanotechnology: Science, Innovation, and Opportunity with a foreword from Senators Joe Lieberman and George Allen, as well as contributions from the world’s leading innovators in nanotechnology.
Currently, Dr. Ho is investigating the fabrication of glucocorticoid-functionalized materials as anti-inflammatory coatings to enhance implant bio-compatibility, as well as using nanowires and nanotubes as neuronal interrogative modalities. Dean’s research achievements have garnered news coverage in
Nature, MICRO/NANO, as well as BBC Radio. He has been invited to speak at several prestigious institutions/events including the Academia Sinica (National Academy of Sciences-Taiwan), California Nanosystems Institute (CNSI), as well as the
Bionanotechnology: Academic and Industrial Partnerships in the UK and US consortium before the Minister of Science and Innovation of the United Kingdom.
Dr. Ho is an Associate Editor of the
Journal of Nanotechnology Law and Business, and was previously a board member of University Camps, Inc., the parent company of the 72 year-old official charity of the University of California, Los Angeles. In addition, for his contributions to the UCLA community, Dr. Ho was presented with the Chancellor’s Service Award in 2002. Dr. Ho is a member of the AAAS, MRS, ASME, BMES, and American Academy of Nanomedicine, and was elected a member of Sigma Xi in 2005.